Autophagy in MCF-7 cancer cells induced by copper complexes
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F16%3A43901835" target="_blank" >RIV/60461373:22330/16:43901835 - isvavai.cz</a>
Result on the web
<a href="http://www.sciencedirect.com/science/article/pii/S1734114016300986" target="_blank" >http://www.sciencedirect.com/science/article/pii/S1734114016300986</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.pharep.2016.07.011" target="_blank" >10.1016/j.pharep.2016.07.011</a>
Alternative languages
Result language
angličtina
Original language name
Autophagy in MCF-7 cancer cells induced by copper complexes
Original language description
Background: Autophagy plays an important role in cancer cells. Targeting autophagy in cancer can provide new opportunities for drug development. Methods: In this study we tested four Schiff base Cu(II) complexes against human breast cancer cells (MCF-7) and human non-cancerous cells (HEK-293T). We have tested their cytotoxic effect by evaluating IC50 using MTT test. To detect morphological changes of the actin fibers we have used fluorescent microscopy. To determine the type of cell death we used electrophoretic analysis and western blot analysis (protein LC3). Results: IC50 values of the complexes increased with time of their influence, indicating acquired resistance of MCF-7 to the complexes. Healthy cells HEK-293T were not sensitive to the Cu(II) complexes. Compared with the control cells (cells without Cu(II) complexes) which were without morphological changes of actin fibers, Cu(II) complexes induced condensation and asymmetric conformational changes in actin filaments. To examine the type of cell death induced by the Cu(II) complexes we treated MCF-7 cells with Cu(II) complexes (1, 10, 50 and 100 mu mol/L) during a 72 h incubation period. By electrophoresis we have not detected any DNA fragmentation. To determine whether Cu(II) complexes induced autophagy or necrotic cell death we used the western blot analysis. MCF-7 cells influenced with tested Cu(II) complexes produced LC3 protein after their 72 h incubation indicating autophagy in MCF-7 cancer cells. Conclusions: Tested Schiff base copper (II) complexes have antiproliferative activity against cancer cells but not against healthy cells. They have induced autophagy in the cancer cell line MCF-7.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FR - Pharmacology and apothecary chemistry
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Pharmacological Reports
ISSN
1734-1140
e-ISSN
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Volume of the periodical
68
Issue of the periodical within the volume
6
Country of publishing house
PL - POLAND
Number of pages
4
Pages from-to
1221-1224
UT code for WoS article
000388432100017
EID of the result in the Scopus database
2-s2.0-84988485480