Alternative Pathways of Cancer Cell Death by Rottlerin: Apoptosis versus Autophagy

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F12%3A%230000927" target="_blank" >RIV/00159816:_____/12:#0000927 - isvavai.cz</a>

Alternative codes found

RIV/68081707:_____/12:00440779

Result on the web

<a href="http://dx.doi.org/10.1155/2012/980658" target="_blank" >http://dx.doi.org/10.1155/2012/980658</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1155/2012/980658" target="_blank" >10.1155/2012/980658</a>

Result language

angličtina

Original language name

Alternative Pathways of Cancer Cell Death by Rottlerin: Apoptosis versus Autophagy

Original language description

Since the ability of cancer cells to evade apoptosis often limits the efficacy of radiotherapy and chemotherapy, autophagy is emerging as an alternative target to promote cell death. Therefore, we wondered whether Rottlerin, a natural polyphenolic compound with antiproliferative effects in several cell types, can induce cell death in MCF-7 breast cancer cells. The MCF-7 cell line is a good model of chemo/radio resistance, being both apoptosis and autophagy resistant, due to deletion of caspase 3 gene, high expression of the antiapoptotic protein Bcl-2, and low expression of the autophagic Beclin-1 protein. The contribution of autophagy and apoptosis to the cytotoxic effects of Rottlerin was examined by light, fluorescence, and electron microscopic examination and by western blotting analysis of apoptotic and autophagic markers. By comparing caspases-3-deficient (MCF-7(3def)) and caspases-3-transfected MCF-7 cells (MCF-7(3trans)), we found that Rottlerin induced a noncanonical, Bcl-2-,

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FP - Other medical fields

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE

ISSN

1741-427X

e-ISSN

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Volume of the periodical

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Issue of the periodical within the volume

listopad

Country of publishing house

GB - UNITED KINGDOM

Number of pages

11

Pages from-to

1-11

UT code for WoS article

000312865700001

EID of the result in the Scopus database

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