The role of individual caspases in cell death induction by taxanes in breast cancer cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F15%3A43909312" target="_blank" >RIV/00216208:11120/15:43909312 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1186/s12935-015-0155-7" target="_blank" >http://dx.doi.org/10.1186/s12935-015-0155-7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s12935-015-0155-7" target="_blank" >10.1186/s12935-015-0155-7</a>

Result language

angličtina

Original language name

The role of individual caspases in cell death induction by taxanes in breast cancer cells

Original language description

Background: In previous study we showed that caspase-2 plays the role of an apical caspase in cell death induction by taxanes in breast cancer cells. This study deals with the role of other caspases. We tested breast cancer cell lines SK-BR-3 (functionalcaspase-3) and MCF-7 (nonfunctional caspase-3). Methods and Results: Using western blot analysis we demonstrated the activation of initiator caspase-8 and -9 as well as executioner caspase-6 and -7 in both tested cell lines after application of taxanes(paclitaxel, SB-T-1216) at death-inducing concentrations. Caspase-3 activation was also found in SK-BR-3 cells. Employing specific siRNAs after taxane application, suppression of caspase-3 expression significantly increased the number of surviving SK-BR-3 cells. Inhibition of caspase-7 expression also increased the number of surviving SK-BR-3 and MCF-7 cells. On the other hand, suppression of caspase-8 and caspase-9 expression had no significant effect on cell survival. However, caspase-

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GA301%2F09%2F0362" target="_blank" >GA301/09/0362: Role of caspase-2 in apoptosis induction by taxanes in breast cancer cells</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cancer Cell International

ISSN

1475-2867

e-ISSN

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Volume of the periodical

15

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

16

Pages from-to

"Article ID 8; 1"-16

UT code for WoS article

000350172000001

EID of the result in the Scopus database

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