Differing Mechanisms of Death Induction by Fluorinated Taxane SB-T-12854 in Breast Cancer Cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F17%3A43913153" target="_blank" >RIV/00216208:11120/17:43913153 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.21873/anticanres.11488" target="_blank" >http://dx.doi.org/10.21873/anticanres.11488</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.21873/anticanres.11488" target="_blank" >10.21873/anticanres.11488</a>

Result language

angličtina

Original language name

Differing Mechanisms of Death Induction by Fluorinated Taxane SB-T-12854 in Breast Cancer Cells

Original language description

Background/Aim: Classical taxanes are routinely used in cancer therapy. In this study, mechanisms involved in death induction by novel fluorine-containing taxane SBT-12854 were investigated. Materials and Methods: We employed breast cancer SK-BR-3, MCF-7 and T47D cell lines to assess activation of individual caspases, changes in the expression of proteins of the Bcl-2 family, and the release of pro-apoptotic factors from mitochondria into the cytosol after SB-T-12854 treatment. Results: Caspase-2, -8, and -9 were activated in SK-BR-3 and MCF-7 cells. Only caspase8 was activated in T47D cells. Caspase-7 and -6 were activated in all tested cells while caspase-3 was activated only in SK-BR-3 cells. Pro-apoptotic Bad protein seems to be important for cell death induction in all tested cells. Antiapoptotic Bcl-2 and pro-apoptotic Bim, Bok, Bid and Bik seem to be also associated with cell death induction in some of the tested cells. The mitochondrial apoptotic pathway was significantly activated in association with the release of cytochrome c and Smac from mitochondria, but only in SKBR-3 cells, not in MCF-7 and T47D cells. Conclusion: Cell death induced by SB-T-12854, in the tested breast cancer cells, differs regarding activation of caspases, changes in levels of pro-apoptotic and anti-apoptotic proteins of the Bcl2 family and activation of the mitochondrial apoptotic pathway.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

—

Continuities

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Anticancer Research

ISSN

0250-7005

e-ISSN

—

Volume of the periodical

37

Issue of the periodical within the volume

4

Country of publishing house

GR - GREECE

Number of pages

10

Pages from-to

1581-1590

UT code for WoS article

000402167700006

EID of the result in the Scopus database

2-s2.0-85017478998