Selection of endogenous control and identification of significant microRNA deregulations in cervical cancer
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F23%3A10464806" target="_blank" >RIV/00179906:_____/23:10464806 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11150/23:10464806
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=IIzf1mEylX" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=IIzf1mEylX</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3389/fonc.2023.1143691" target="_blank" >10.3389/fonc.2023.1143691</a>
Alternative languages
Result language
angličtina
Original language name
Selection of endogenous control and identification of significant microRNA deregulations in cervical cancer
Original language description
IntroductionCervical cancer causes approximately 350,000 deaths each year. The availability of sensitive and specific diagnostic tests to detect cervical cancer in its early stages is essential to improve survival rates. MethodsIn this study, we compared two strategies for selecting endogenous controls: miRNA profiling by small-RNA sequencing and a commercially available microfluidic card with 30 recommended endogenous controls preloaded by the manufacturer. We used the RefFinder algorithm and coefficient of variation to select endogenous controls. We selected the combination of miR-181a-5p and miR-423-3p as the most optimal normalizer. In the second part of this study, we determined the differential expression (between tumor/non-tumor groups) of microRNA in cervical cancer FFPE tissue samples. We determined the comprehensive miRNA expression profile using small-RNA sequencing technology and verified the results by real-time PCR. We determined the relative expression of selected miRNAs using the 2(-Delta Delta Ct) method. ResultsWe detected statistically significant upregulation of miR-320a-3p, miR-7704, and downregulation of miR-26a-5p in the tumor group compared to the control group. The combination of these miRNAs may have the potential to be utilized as a diagnostic panel for cervical cancer. Using ROC curve analysis, the proposed panel showed 93.33% specificity and 96.97% sensitivity with AUC = 0.985. ConclusionsWe proposed a combination of miR-181a-5p and miR-423-3p as optimal endogenous control and detected potentially significant miRNAs (miR-320a-3p, miR-7704, miR-26a-5p). After further validation of our results, these miRNAs could be used in a diagnostic panel for cervical cancer.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30204 - Oncology
Result continuities
Project
<a href="/en/project/EF16_013%2F0001674" target="_blank" >EF16_013/0001674: BBMRI-CZ: Biobank network - a versatile platform for the research of the etiopathogenesis of diseases</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Frontiers in Oncology
ISSN
2234-943X
e-ISSN
2234-943X
Volume of the periodical
13
Issue of the periodical within the volume
APR
Country of publishing house
CH - SWITZERLAND
Number of pages
9
Pages from-to
1143691
UT code for WoS article
000982675400001
EID of the result in the Scopus database
2-s2.0-85158144958