Unsolved mystery of Fas: mononuclear cells may have trouble dying in patients with Sjogren's syndrome
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F23%3A10466207" target="_blank" >RIV/00179906:_____/23:10466207 - isvavai.cz</a>
Alternative codes found
RIV/44555601:13440/23:43897743 RIV/00216208:11150/23:10466207
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=Nj4N7HCFJ5" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=Nj4N7HCFJ5</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/s12865-023-00544-5" target="_blank" >10.1186/s12865-023-00544-5</a>
Alternative languages
Result language
angličtina
Original language name
Unsolved mystery of Fas: mononuclear cells may have trouble dying in patients with Sjogren's syndrome
Original language description
Background: Patients with Sjogren's syndrome, like other patients with autoimmune disorders, display dysregulation in the function of their immune system. Fas and Fas Ligand (FasL) are among the dysregulated proteins. Methods: We studied Fas and FasL on IL-2R alpha(+) cells and in serum of patients with Sjogren's syndrome (n = 16) and healthy individuals (n = 16); both from same ethnic and geographical background. We used flow cytometry and enzyme-linked immunosorbent for this purpose. We also measured the expression of Bcl-2 and Bax by reverse transcription quantitative real-time PCR (RT-qPCR) and percentage of apoptotic and dead cells using Annexin V and 7-AAD staining in lymphocytes. Results: FasL was increased in patients' T and B cells while Fas was increased in patients' monocytes, T and B cells. No signs of increased apoptosis were found. sFas and sFasL in patients' serum were increased, although the increase in sFasL was not significant. We suspect an effect of non-steroidal anti-inflammatory therapy on B cells, explaining the decrease of the percentage Fas(+) B cells found within our samples. In healthy individuals, there was a noticeable pattern in the expression of FasL which mutually correlated to populations of mononuclear cells; this correlation was absent in the patients with Sjogren's syndrome. Conclusion: sMononuclear cells expressing IL-2R alpha(+) had upregulated Fas in Sjogren's syndrome. However, the rate of apoptosis based on Annexin V staining and the Bcl-2/Bax expression was not observed in mononuclear cells. We suspect a functional role of abnormal levels of Fas and FasL which has not been cleared yet.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30208 - Dentistry, oral surgery and medicine
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
BMC Immunology
ISSN
1471-2172
e-ISSN
1471-2172
Volume of the periodical
24
Issue of the periodical within the volume
1
Country of publishing house
GB - UNITED KINGDOM
Number of pages
15
Pages from-to
12
UT code for WoS article
001011253300001
EID of the result in the Scopus database
2-s2.0-85162754222