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Unsolved mystery of Fas: mononuclear cells may have trouble dying in patients with Sjogren's syndrome

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F23%3A10466207" target="_blank" >RIV/00179906:_____/23:10466207 - isvavai.cz</a>

  • Alternative codes found

    RIV/44555601:13440/23:43897743 RIV/00216208:11150/23:10466207

  • Result on the web

    <a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=Nj4N7HCFJ5" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=Nj4N7HCFJ5</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1186/s12865-023-00544-5" target="_blank" >10.1186/s12865-023-00544-5</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Unsolved mystery of Fas: mononuclear cells may have trouble dying in patients with Sjogren's syndrome

  • Original language description

    Background: Patients with Sjogren&apos;s syndrome, like other patients with autoimmune disorders, display dysregulation in the function of their immune system. Fas and Fas Ligand (FasL) are among the dysregulated proteins. Methods: We studied Fas and FasL on IL-2R alpha(+) cells and in serum of patients with Sjogren&apos;s syndrome (n = 16) and healthy individuals (n = 16); both from same ethnic and geographical background. We used flow cytometry and enzyme-linked immunosorbent for this purpose. We also measured the expression of Bcl-2 and Bax by reverse transcription quantitative real-time PCR (RT-qPCR) and percentage of apoptotic and dead cells using Annexin V and 7-AAD staining in lymphocytes. Results: FasL was increased in patients&apos; T and B cells while Fas was increased in patients&apos; monocytes, T and B cells. No signs of increased apoptosis were found. sFas and sFasL in patients&apos; serum were increased, although the increase in sFasL was not significant. We suspect an effect of non-steroidal anti-inflammatory therapy on B cells, explaining the decrease of the percentage Fas(+) B cells found within our samples. In healthy individuals, there was a noticeable pattern in the expression of FasL which mutually correlated to populations of mononuclear cells; this correlation was absent in the patients with Sjogren&apos;s syndrome. Conclusion: sMononuclear cells expressing IL-2R alpha(+) had upregulated Fas in Sjogren&apos;s syndrome. However, the rate of apoptosis based on Annexin V staining and the Bcl-2/Bax expression was not observed in mononuclear cells. We suspect a functional role of abnormal levels of Fas and FasL which has not been cleared yet.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    30208 - Dentistry, oral surgery and medicine

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2023

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    BMC Immunology

  • ISSN

    1471-2172

  • e-ISSN

    1471-2172

  • Volume of the periodical

    24

  • Issue of the periodical within the volume

    1

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    15

  • Pages from-to

    12

  • UT code for WoS article

    001011253300001

  • EID of the result in the Scopus database

    2-s2.0-85162754222