Dried urine spot as a stable, green, and practical microsampling tool in clinical practice for quantification of neopterin and creatinine
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F25%3A10499513" target="_blank" >RIV/00179906:_____/25:10499513 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11160/25:10499513
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=wU9W7s1x0a" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=wU9W7s1x0a</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1039/d5gc00956a" target="_blank" >10.1039/d5gc00956a</a>
Alternative languages
Result language
angličtina
Original language name
Dried urine spot as a stable, green, and practical microsampling tool in clinical practice for quantification of neopterin and creatinine
Original language description
Dried urine spot (DUS) is demonstrated to be a useful microsampling technique for neopterin and creatinine analysis. The method was verified using samples from 12 healthy volunteers, 12 cancer patients, and 12 patients infected with the SARS-CoV-2 virus and provided results comparable to the routine reference method. DUS samples were prepared using a simple dilute-and-shoot approach with a buffer solution prior to analysis. The developed method requires only 10 mu L samples, making it feasible for home sampling when combined with commercially available pre-cut DUS devices with volumetric microfluidics or capillary transfer tubes. The method is environmentally friendly as it minimizes reagent use, requires only 110 mu L phosphate buffer and no organic solvents. DUS also requires 250 times less storage space than liquid urine. The technique also improves laboratory work safety by minimizing the use of harmful extraction solvents. Dried samples are non-biohazardous and stable for up to 5 days at 40 degrees C and for 4 months at room temperature. This allows cost-effective transport via standard mail. The study also evaluated practical considerations for clinical laboratories and home sampling. Volumetric urine sampling is simple using capillary transfer tubes or commercially available volumetric microfluidic devices. When integrated with telemedicine, this microsampling approach can reduce unnecessary hospital visits for patients and improve patient access to clinical testing.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
30202 - Endocrinology and metabolism (including diabetes, hormones)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Green Chemistry
ISSN
1463-9262
e-ISSN
1463-9270
Volume of the periodical
27
Issue of the periodical within the volume
36
Country of publishing house
GB - UNITED KINGDOM
Number of pages
14
Pages from-to
11007-11020
UT code for WoS article
001522484900001
EID of the result in the Scopus database
2-s2.0-105009945451