Volumetric dried blood spot microsampling: A sustainable, patient-friendly, and practical approach for retinol and alpha-tocopherol analysis in a clinical setting
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F25%3A10504947" target="_blank" >RIV/00179906:_____/25:10504947 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11160/25:10504947
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=_R-qUHvILD" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=_R-qUHvILD</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ymeth.2025.08.006" target="_blank" >10.1016/j.ymeth.2025.08.006</a>
Alternative languages
Result language
angličtina
Original language name
Volumetric dried blood spot microsampling: A sustainable, patient-friendly, and practical approach for retinol and alpha-tocopherol analysis in a clinical setting
Original language description
Dried blood spot (DBS) technique has gained significant attention due to the growth of decentralized diagnostics. This technique reduces the number of hospital visits for patients and the workload for personnel in specialized hospitals. This microsampling method provides an environmentally friendly (green) and patient-friendly alternative to conventional phlebotomy. Challenges related to sample heterogeneity in traditional DBS cards have been overcome by the volumetric DBS sampling using new types of commercially available devices. Due to the unstable nature of the analytes, commercial volumetric DBS devices allow blood sampling at primary care units in remote settings and facilitate transport it via temperature-controlled systems. Blood sample stability has improved from 24 h at 4-8 degrees C to 30 days at -80 degrees C. DBS also requires over 1000 times less shipping and storage space than liquid blood. We optimized the DBS method to require only 10 mu L of blood and achieve extraction efficiencies of over 90 % for retinol when the result from validated method is the reference value. However, alpha-tocopherol recovery varied from 53 to 75 % depending on the filter paper type used. Furthermore, we successfully developed a liquid-liquid extraction method for both analytes from whole blood, with over 90 % recovery. Our approaches eliminate the need for separate serum and erythrocyte extractions, simplify sample preparation, and reduce reagent use and energy consumption. Both devices enable reliable volumetric collection. Our approach makes micronutrient monitoring more accessible and enables sample collection in decentralized settings. This aligns with the objectives of green analytical chemistry and universal health coverage.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30104 - Pharmacology and pharmacy
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Methods
ISSN
1046-2023
e-ISSN
1095-9130
Volume of the periodical
243
Issue of the periodical within the volume
NOV
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
40-48
UT code for WoS article
001561944800001
EID of the result in the Scopus database
2-s2.0-105014031175