Assessment of DNA damage of smooth muscle cells in tunica media of human arterial allografts using Comet assay method
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F25%3A10504795" target="_blank" >RIV/00179906:_____/25:10504795 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11110/25:10504795 RIV/00216208:11150/25:10504795 RIV/00023001:_____/25:00085944 RIV/00064165:_____/25:10504795
Result on the web
<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=2oQx.avNI4" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=2oQx.avNI4</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1371/journal.pone.0335044" target="_blank" >10.1371/journal.pone.0335044</a>
Alternative languages
Result language
angličtina
Original language name
Assessment of DNA damage of smooth muscle cells in tunica media of human arterial allografts using Comet assay method
Original language description
During the harvest and subsequent processing of blood vessels for allogeneic transplantation, DNA damage can occur. A suitable method for quantifying this damage may be the comet assay, also known as the single cell gel electrophoresis assay, which is used to measure DNA damage at the single-cell level. We evaluated the key indicators of muscle cell DNA damage in the tunica media of arteries by using the comet assay with four groups: (1) cryopreserved grafts thawed slowly, (2) cryopreserved grafts thawed rapidly, (3) cold-preserved "fresh" grafts harvested as part of a multiorgan procurement, and (4) cadaveric arterial graft harvested at autopsy. Kruskal-Wallis multiple-comparison testing identified a statistically significant difference between rapidly thawed grafts and multiorgan harvested fresh grafts. Evans' correlation criterion indicated a moderately strong correlation between the percentage of DNA in the comet head and warm ischemia duration in the group of slowly thawed grafts, and between the percentage of DNA in the comet head and cold ischemia duration in the group of cadaveric grafts. No association of come assay parameters with age was demonstrated. Comet assay showed that the cryopreservation and storage processes described in this study did not lower the DNA content in comparison with fresh grafts collected during multiorgan harvests in operating rooms and that DNA content was not influenced by the type of thawing protocol.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30213 - Transplantation
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
PLoS One
ISSN
1932-6203
e-ISSN
1932-6203
Volume of the periodical
20
Issue of the periodical within the volume
11
Country of publishing house
US - UNITED STATES
Number of pages
16
Pages from-to
e0335044
UT code for WoS article
001607959300027
EID of the result in the Scopus database
2-s2.0-105020642377