Evaluation of fluorescent capillary electrophoresis for rapid identification of Candida fungal infections

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209775%3A_____%2F16%3AN0000016" target="_blank" >RIV/00209775:_____/16:N0000016 - isvavai.cz</a>

Result on the web

<a href="http://jcm.asm.org/content/54/5/1295.full.pdf+html" target="_blank" >http://jcm.asm.org/content/54/5/1295.full.pdf+html</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1128/JCM.00118-16" target="_blank" >10.1128/JCM.00118-16</a>

Result language

angličtina

Original language name

Evaluation of fluorescent capillary electrophoresis for rapid identification of Candida fungal infections

Original language description

Early diagnosis of fungal infection is critical for initiating antifungal therapy and reducing the high mortality rate in immunocompromised patients. In this study, we focused on rapid and sensitive identification of clinically important Candida species, utilizing the variability in the length of the ITS2 rRNA gene and fluorescent capillary electrophoresis (f-ITS2-PCR-CE). The method was developed and optimized on 29 various Candida reference strains from which 26 Candida species were clearly identified, while Candida guilliermondii, C. fermentati, and C. carpophila, which are closely related, could not be distinguished. The method was subsequently validated on 143 blinded monofungal clinical isolates (comprising 26 species) and was able to identify 88% of species unambiguously. This indicated a higher resolution power than the classical phenotypic approach which correctly identified 73%. Finally, the culture-independent potential of this technique was addressed by the analysis of 55 retrospective DNA samples extracted directly from clinical material. The method showed 100% sensitivity and specificity compared to those of the combined results of cultivation and panfungal PCR followed by sequencing used as a gold standard. In conclusion, this newly developed f-ITS2-PCR-CE analytical approach was shown to be a fast, sensitive, and highly reproducible tool for both cul- ture-dependent and culture-independent identification of clinically important Candida strains, including species of the “psilosis” complex.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30303 - Infectious Diseases

Project

<a href="/en/project/NT13242" target="_blank" >NT13242: Poly-microbial biofilm - the cause of chronic urinary infections and therapy failure: cultivation and PCR-DGGE diagnostics, importance and possibilities of affection</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Clinical Microbiology

ISSN

0271-9142

e-ISSN

—

Volume of the periodical

54

Issue of the periodical within the volume

5

Country of publishing house

US - UNITED STATES

Number of pages

9

Pages from-to

1295-1303

UT code for WoS article

000374955700021

EID of the result in the Scopus database

2-s2.0-84964981045