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Rapid detection of fungal pathogens in bronchoalveolar lavage samples using panfungal PCR combined with high resolution melting analysis
at panfungal PCR as an IFD diagnostic tool from BAL samples. The applicability and accurate pathogen identification is necessary. We designed a panfungal polymerase chain reaction (PCR), which targets the highly v...
FN - Epidemiologie, infekční nemoci a klinická imunologie
- 2016 •
- Jx •
- Link
Rok uplatnění
Jx - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
Výsledek na webu
Detection and identification of fungi in bronchoalveolar lavage fluid from immunocompromised patients using panfungal PCR
described as uncultured fungus. Panfungal PCR detected fungal species that would be missed by specific methods in only one case (probable cryptococcosis). Panfungal PCR are still challenging. In this study, we use...
Microbiology
- 2019 •
- Jimp •
- Link
Rok uplatnění
Jimp - Článek v periodiku v databázi Web of Science
Výsledek na webu
Rapid sourdough yeast identification using panfungal PCR combined with High Resolution Melting analysis
identification of sourdough yeasts. We designed a panfungal real time-PCR targeting the ITS2 panfungal PCR combined with High Resolution Melting analysis. Ostatní – Poster a sborník was used to assess the sourdoug...
Agricultural biotechnology and food biotechnology
- 2022 •
- O
Rok uplatnění
O - Ostatní výsledky
Prevalence of fungal DNAemia mediated by putatively non-pathogenic fungi in immunocompromised patients with febrile neutropenia: a prospective cohort study
allogeneic hematopoietic stem cell transplantation. Two different panfungal-PCR-screening exploit the diagnostic potential of panfungal-PCR approaches for detecting IFD by ensuing analysis. If applied appropriatel...
Hematology
- 2024 •
- Jimp •
- Link
Rok uplatnění
Jimp - Článek v periodiku v databázi Web of Science
Výsledek na webu
Kit for detection and identification of mycotic pathogens
Despite advances in the treatment of invasive fungal diseases, mortality rates remain high. Moreover, due to the expanding spectrum of causative agents, fast and accurate pathogen identification is necessary. We designed a panfungal PCR<...
FD - Onkologie a hematologie
- 2016 •
- Fuzit •
- Link
Rok uplatnění
Fuzit - Užitný vzor
Výsledek na webu
Rapid sourdough yeast identification using panfungal PCR combined with high resolution melting analysis
identification of sourdough yeasts. We designed a panfungal real time-PCR targeting the ITS2 was used to assess the sourdough yeasts. Additionally, using the HRM-PCR method the contamination with the ergot fungus DNA was r...
Agricultural biotechnology and food biotechnology
- 2022 •
- Jimp •
- Link
Rok uplatnění
Jimp - Článek v periodiku v databázi Web of Science
Výsledek na webu
Evaluation of Fluorescent Capillary Electrophoresis for Rapid Identification of Candida Fungal Infections
and panfungal PCR followed by sequencing used as a gold standard. In conclusion, this newly developed f-ITS2-PCR-CE analytical approach was shown to be a fast, sensitive and fluorescent capillary electrophoresis (f-ITS2-
EE - Mikrobiologie, virologie
- 2016 •
- Jx •
- Link
Rok uplatnění
Jx - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
Výsledek na webu
Evaluation of fluorescent capillary electrophoresis for rapid identification of Candida fungal infections
and panfungal PCR followed by sequencing used as a gold standard. In conclusion, this newly developed f-ITS2-PCR-CE analytical approach was shown to be a fast, sensitive and fluorescent capillary electrophoresis (f-ITS2-
Infectious Diseases
- 2016 •
- Jimp •
- Link
Rok uplatnění
Jimp - Článek v periodiku v databázi Web of Science
Výsledek na webu
Fluorescent Capillary Electrophoresis Is Superior to Culture in Detecting Candida Species from Samples of Urinary Catheters and Ureteral Stents with Mono- or Polyfungal Biofilm Growth
by fluorescent capillary electrophoresis (f-ITS2-PCR-CE) and to compare the results (26.5%), and Candida tropicalis (12.4%) predominating. f-ITS2-PCR-CE was evaluated sensitivity considerably improved to 1.7 times lower than that o...
Microbiology
- 2019 •
- Jimp •
- Link
Rok uplatnění
Jimp - Článek v periodiku v databázi Web of Science
Výsledek na webu
The use of molecular techniques RT-PCR and QRT-PCR for the detection and identification of Potato Leafroll Virus (PLRV) isolates
Fifty-five isolates of PLRV from in-vitro collection (PRI Havlíčkův Brod) were tested by means of RT-PCR and QRT-PCR. The results of QRT-PCR and RT-PCR were compared with ELISA....
GF - Choroby, škůdci, plevely a ochrana rostlin
- 2015 •
- O
Rok uplatnění
O - Ostatní výsledky
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