Impact of acceptor splice site NAGTAG motif on exon recognition
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209775%3A_____%2F19%3AN0000013" target="_blank" >RIV/00209775:_____/19:N0000013 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14110/19:00108505
Result on the web
<a href="https://link.springer.com/article/10.1007%2Fs11033-019-04734-6" target="_blank" >https://link.springer.com/article/10.1007%2Fs11033-019-04734-6</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11033-019-04734-6" target="_blank" >10.1007/s11033-019-04734-6</a>
Alternative languages
Result language
angličtina
Original language name
Impact of acceptor splice site NAGTAG motif on exon recognition
Original language description
Pre-mRNA splicing is an essential step in gene expression, when introns are removed and exons joined by the complex of proteins called spliceosome. Correct splicing requires a precise exon/intron junction definition, which is determined by a consensual donor and acceptor splice site at the 5′ and 3′ end, respectively. An acceptor splice site (3′ss) consists of highly conserved AG nucleotides in positions E−2 and E−1. These nucleotides can appear in tandem, located 3 bp from each other. Then they are referred to as NAGNAG or tandem 3′ss, which can be alternatively spliced. NAG/TAG 3′ss motif abundance is extremely low and cannot be easily explained by just a nucleotide preference in this position. We tested artificial NAG/TAG motif’s potential negative effect on exon recognition using a minigene assay. Introducing the NAG/TAG motif into seven different exons revealed no general negative effect on exon recognition. The only observed effect was the partial use of the newly formed distal 3′ss. We can conclude that this motif’s extremely low preference in a natural 3′ss is not a consequence of the NAG/TAG motif’s negative effect on exon recognition, but more likely the result of other RNA processing aspects, such as an alternative 3′ss choice, decreased 3′ss strength, or incorporating an amber stop codon.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
<a href="/en/project/NV16-34414A" target="_blank" >NV16-34414A: Determination of genetic regions susceptible to splicing affecting mutations</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Molecular Biology Reports
ISSN
0301-4851
e-ISSN
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Volume of the periodical
46
Issue of the periodical within the volume
3
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
8
Pages from-to
2877-2884
UT code for WoS article
000470332600028
EID of the result in the Scopus database
2-s2.0-85062700137