Nucleotides in both donor and acceptor splice sites are responsible for choice in NAGNAG tandem splice sites
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209775%3A_____%2F21%3AN0000012" target="_blank" >RIV/00209775:_____/21:N0000012 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14110/21:00123981
Result on the web
<a href="https://link.springer.com/article/10.1007/s00018-021-03943-2" target="_blank" >https://link.springer.com/article/10.1007/s00018-021-03943-2</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00018-021-03943-2" target="_blank" >10.1007/s00018-021-03943-2</a>
Alternative languages
Result language
angličtina
Original language name
Nucleotides in both donor and acceptor splice sites are responsible for choice in NAGNAG tandem splice sites
Original language description
Among alternative splicing events in the human transcriptome, tandem NAGNAG acceptor splice sites represent an appreciable proportion. Both proximal and distal NAG can be used to produce two splicing isoforms differing by three nucleotides. In some cases, the upstream exon can be alternatively spliced as well, which further increases the number of possible transcripts. In this study, we showed that NAG choice in tandem splice site depends considerably not only on the concerned acceptor, but also on the upstream donor splice site sequence. Using an extensive set of experiments with systematically modified two-exonic minigene systems of AFAP1L2 or CSTD gene, we recognized the third and fifth intronic upstream donor splice site position and the tandem acceptor splice site region spanning from −10 to +2, including NAGNAG itself, as the main drivers. In addition, competition between different branch points and their composition were also shown to play a significant role in NAG choice. All these nucleotide effects appeared almost additive, which explained the high variability in proximal versus distal NAG usage.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
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Continuities
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Cellular and Molecular Life science
ISSN
1420-682X
e-ISSN
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Volume of the periodical
78
Issue of the periodical within the volume
21-22
Country of publishing house
CH - SWITZERLAND
Number of pages
15
Pages from-to
6979-6993
UT code for WoS article
000702592500001
EID of the result in the Scopus database
2-s2.0-85116039158