Specific Modulation of p53 Binding to Supercoiled DNA with and without Consensus Sequence by Monoclonal Antibodies

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F00%3A00000103" target="_blank" >RIV/00209805:_____/00:00000103 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Specific Modulation of p53 Binding to Supercoiled DNA with and without Consensus Sequence by Monoclonal Antibodies

Original language description

The wild type human p53 protein binds preferentially to supercoiled DNA in vitro both in the presence and absence of p53 consensus sequence. The p53-DNA binding is decreased by physiological concentrations of zinc ions and at higher zinc concentrations the binding of p53 to sc DNA is completely inhibited. In contrast, oxidation of the protein with diamide resulted in partial inhibition of p53 - scDNA binding. The activation of p53 for sequence-specific binding within scDNA was also studied. C-terminal specific antibody Bp53-10 activated the sequence-specific binding of p53 to p53CON within pPGM1 scDNA but did not influence its binding to pBluescript scDNA.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

FD - Oncology and haematology

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2000

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

10th International p53 Workshop

ISBN

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ISSN

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e-ISSN

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Number of pages

1

Pages from-to

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Publisher name

neuv.

Place of publication

Monterey

Event location

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Event date

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Type of event by nationality

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UT code for WoS article

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