Divergent substrate-binding loop within the pro-oncogenic protein anterior gradient-2 forms a docking site for reptin

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F10%3A%230000597" target="_blank" >RIV/00209805:_____/10:#0000597 - isvavai.cz</a>
Alternative codes found
RIV/00209805:_____/10:#0000680
Result on the web
<a href="http://www.sciencedirect.com/science/article/pii/S0022283610010235" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0022283610010235</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jmb.2010.09.035" target="_blank" >10.1016/j.jmb.2010.09.035</a>

Result language
angličtina
Original language name
Divergent substrate-binding loop within the pro-oncogenic protein anterior gradient-2 forms a docking site for reptin
Original language description
Anterior gradient-2 (AGR2) functions in a range of biological systems, including goblet cell formation, limb regeneration, inhibition of p53, and metastasis. There are no well-validated binding proteins for AGR2 protein despite the wealth of data implicating an important cellular function in vertebrates. The yeast two-hybrid system was used to isolate the ATP binding protein Reptin as an AGR2-interacting protein. AGR2 formed a stable complex in human cell lysates with Reptin, thus validating Reptin as an AGR2 binding protein in cells. Reptin was also shown to be overproduced in a panel of primary breast cancer biopsy specimens, relative to normal adjacent tissue from the same patient, suggesting a role in cancer growth in vivo. Mutations were made at the two ATP binding motifs in Reptin to evaluate the effects of ATP on Reptin?AGR2 complex stability. Loss-of-ATP binding mutations at the Walker A motif (K83A) or gain-of- ATP binding mutations at the Walker B motif (D299N) resulted in Re
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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