Novel entropically driven conformation-specific interactions with Tomm34 modulate Hsp70 folding and ATPase activities

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F16%3AN0000021" target="_blank" >RIV/00209805:_____/16:N0000021 - isvavai.cz</a>
Result on the web
<a href="http://www.mcponline.org/content/15/5/1710.long" target="_blank" >http://www.mcponline.org/content/15/5/1710.long</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1074/mcp.M116.058131" target="_blank" >10.1074/mcp.M116.058131</a>

Result language
angličtina
Original language name
Novel entropically driven conformation-specific interactions with Tomm34 modulate Hsp70 folding and ATPase activities
Original language description
Co-chaperones containing TPR domains enable cooperation between Hsp70 and Hsp90 to maintain cellular proteostasis. Whilst details of the molecular interactions between some TPR domains and Hsps are known, we describe a novel mechanism by which Tomm34 interacts with and coordinates Hsp70 activities. In contrast to previously defined Hop, Tomm34 interaction is dependent on the Hsp70 chaperone cycle. Tomm34 binds Hsp70 in a complex process: anchorage of the Hsp70 C-terminus by TPR1 domain is accompanied by additional contacts formed exclusively in ATP-bound state of Hsp70 resulting in a high-affinity entropically driven interaction. Tomm34 induces structural changes in determinants within the Hsp70-lid subdomain, modulates Hsp70/Hsp40-mediated refolding and Hsp40-stimulated Hsp70 ATPase activity. Since Tomm34 recruits Hsp90 through its TPR2 domain, we propose a model in which Tomm34 enables Hsp70/Hsp90 scaffolding and influences the Hsp70 chaperone cycle, providing an additional role for co-chaperones that contain multiple TPR domains in regulating protein homeostasis.
Czech name
—
Czech description
—

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
—

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)