Electrochemical sensing of interaction of anterior gradient-2 protein with peptides at a charged interface
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F18%3A00077979" target="_blank" >RIV/00209805:_____/18:00077979 - isvavai.cz</a>
Alternative codes found
RIV/68081707:_____/18:00492336
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0013468618304663" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0013468618304663</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.electacta.2018.02.152" target="_blank" >10.1016/j.electacta.2018.02.152</a>
Alternative languages
Result language
angličtina
Original language name
Electrochemical sensing of interaction of anterior gradient-2 protein with peptides at a charged interface
Original language description
Anterior gradient-2 protein (AGR2) is overexpressed in many human cancers, and this protein presents a novel promising cancer biomarker. We show significant progress in understanding of the electric field effects on AGR2-peptides complexes using constant current chronopotentiometric stripping (CPS) analysis. Surface-attached AGR2-peptide complexes can be disintegrated as a result of their exposure to negative potentials. By controlling the exposure time and temperature, peaks of weakly bound nonspecific complexes can be discriminated from tightly bound specific complexes. Using CPS analysis we found that mutant E60A-AGR2 forms weaker complex with peptide aptamer in comparison to wild type AGR2. These data highlight the utility of this method for studying real-time dynamics of surface-attached protein-peptide complexes.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Electrochimica Acta
ISSN
0013-4686
e-ISSN
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Volume of the periodical
269
Issue of the periodical within the volume
10.4.2018
Country of publishing house
GB - UNITED KINGDOM
Number of pages
75
Pages from-to
70
UT code for WoS article
000428806700009
EID of the result in the Scopus database
2-s2.0-85042718704