Protein structural transition at negatively charged electrode surfaces. Effects of temperature and current density

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F15%3A00447601" target="_blank" >RIV/68081707:_____/15:00447601 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.electacta.2015.06.009" target="_blank" >http://dx.doi.org/10.1016/j.electacta.2015.06.009</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.electacta.2015.06.009" target="_blank" >10.1016/j.electacta.2015.06.009</a>

Result language
angličtina
Original language name
Protein structural transition at negatively charged electrode surfaces. Effects of temperature and current density
Original language description
Earlier it was shown that surface-attached bovine serum albumin undergoes ionic strength-induced structural transition at mercury electrodes. Using constant current chronopotentiometric stripping (CPS) here we show that this electric field-driven transition is influenced also by other factors, such as temperature, current density, and particularly by the time of surface-attached protein exposure to the negative potentials. For example, 1 s exposure to -1.6 V (against Ag/AgC1/3 M KC1) results in the protein denaturation. Exposures longer than 1 s resulted in protein denaturation even at less negative potentials. On the other hand, in CPS much shorter exposure times can be used. Exposing the surface-attached protein to negative potentials for ms time intervals the protein stability can be tested at different current densities. We investigated several proteins such as bovine and human serum albumins, ovalbumin, ot-macroglobulin and ot-synuclein, which showed various profiles in dependence
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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