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MicroRNA isolation and quantification in cerebrospinal fluid: A comparative methodical study

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F18%3A00078109" target="_blank" >RIV/00209805:_____/18:00078109 - isvavai.cz</a>

  • Alternative codes found

    RIV/65269705:_____/18:00069232 RIV/00216224:14740/18:00106965

  • Result on the web

    <a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6285981/pdf/pone.0208580.pdf" target="_blank" >https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6285981/pdf/pone.0208580.pdf</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1371/journal.pone.0208580" target="_blank" >10.1371/journal.pone.0208580</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    MicroRNA isolation and quantification in cerebrospinal fluid: A comparative methodical study

  • Original language description

    Associated with the pathogenesis of many cancers, including brain tumors, microRNAs (miRNAs) present promising diagnostic biomarkers. These molecules have been also studied in cerebrospinal fluid (CSF), showing great potential as a diagnostic tool in patients with brain tumors. Even though there are some biological and technological factors that could affect the results and their biological and clinical interpretation, miRNA analysis in CSF is not fully standardized. This study aims to compare several RNA extraction and miRNA quantification approaches, including high-throughput technologies and individual miRNA detection methods, thereby contributing to the optimization and standardization of quantification of extracellular miRNAs in CSF. Such knowledge is essential for the potential use of miRNAs as diagnostic biomarkers in brain tumors.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    30204 - Oncology

Result continuities

  • Project

    <a href="/en/project/NV15-34553A" target="_blank" >NV15-34553A: Usage of cerebrospinal fluid microRNAs in diagnosis of brain tumors</a><br>

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2018

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    PLoS ONE [online]

  • ISSN

    1932-6203

  • e-ISSN

  • Volume of the periodical

    13

  • Issue of the periodical within the volume

    12

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    12

  • Pages from-to

    "e0208580"

  • UT code for WoS article

    000452640900023

  • EID of the result in the Scopus database

    2-s2.0-85058123970