AGR2 silencing contributes to metformin-dependent sensitization of colorectal cancer cells to chemotherapy
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F19%3A00078210" target="_blank" >RIV/00209805:_____/19:00078210 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14110/19:00108044
Result on the web
<a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6781747/" target="_blank" >https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6781747/</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3892/ol.2019.10800" target="_blank" >10.3892/ol.2019.10800</a>
Alternative languages
Result language
angličtina
Original language name
AGR2 silencing contributes to metformin-dependent sensitization of colorectal cancer cells to chemotherapy
Original language description
There is growing epidemiological evidence indicating an association between diabetes mellitus and the increased incidence of colorectal cancer (CRC). The preferred initial and most widely used pharmacological agent for the treatment of type 2 diabetes is metformin, which in parallel reduces the risk of CRC and improves patient prognosis. AMP-activated protein kinase (AMPK) appears to be tightly associated with the beneficial metabolic effects of metformin, serving as a cellular energy sensor activated in response to a variety of conditions that deplete cellular energy levels. Such conditions include nutrient starvation (particularly glucose), hypoxia and exposure to toxins that inhibit the mitochondrial respiratory chain complex. The aim of the present study was to determine the effect of metformin on CRC cell lines, with different levels of anterior gradient 2 (AGR2) expression, exposed to 5-fluorouracil (5-FU) and oxaliplatin, alone or in combination with metformin. AGR2 has recently emerged as a factor involved in colon carcinogenesis. In AGR2-knockout cells, markedly higher levels of phosphorylated-AMPK were observed in comparison with control cells transfected with GFP-scrambled guide RNA, which indicated that the presence of AGR2 may interfere with the metformin-dependent activation of AMPK. In addition, metformin in combination with 5-FU and oxaliplatin induced ROS production and attenuated autophagy. This effect was enhanced in AGR2-knockout cells.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30204 - Oncology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Oncology letters
ISSN
1792-1074
e-ISSN
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Volume of the periodical
18
Issue of the periodical within the volume
5
Country of publishing house
GR - GREECE
Number of pages
10
Pages from-to
4964-4973
UT code for WoS article
000503219600064
EID of the result in the Scopus database
2-s2.0-85073713558