Proteomics Identification and Validation of Desmocollin-1 and Catechol-O-Methyltransferase as Proteins Associated with Breast Cancer Cell Migration and Metastasis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F19%3A00078267" target="_blank" >RIV/00209805:_____/19:00078267 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14310/19:00108076 RIV/00159816:_____/19:00072467

Result on the web

<a href="https://onlinelibrary.wiley.com/doi/pdf/10.1002/pmic.201900073" target="_blank" >https://onlinelibrary.wiley.com/doi/pdf/10.1002/pmic.201900073</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/pmic.201900073" target="_blank" >10.1002/pmic.201900073</a>

Result language

angličtina

Original language name

Proteomics Identification and Validation of Desmocollin-1 and Catechol-O-Methyltransferase as Proteins Associated with Breast Cancer Cell Migration and Metastasis

Original language description

Biological treatment of many cancers currently targets membrane bound receptors located on a cell surface. To identify novel membrane proteins associated with migration and metastasis of breast cancer cells, a more migrating subpopulation of MDA-MB-231 breast cancer cell line is selected and characterized. A high-resolution quantitative mass spectrometry with SILAC labeling is applied to analyze their surfaceome and it is compared with that of parental MDA-MB-231 cells. Among 824 identified proteins (FDR &lt; 0.01), 128 differentially abundant cell surface proteins with at least one transmembrane domain are found. Of these, i) desmocollin-1 (DSC1) is validated as a protein connected with lymph node status of luminal A breast cancer, tumor grade, and Her-2 status by immunohistochemistry in the set of 96 primary breast tumors, and ii) catechol-O-methyltransferase is successfully verified as a protein associated with lymph node metastasis of triple negative breast cancer as well as with tumor grade by targeted data extraction from the SWATH-MS data of the same set of tissues. The findings indicate importance of both proteins for breast cancer development and metastasis and highlight the potential of biomarker validation strategy via targeted data extraction from SWATH-MS datasets.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Proteomics

ISSN

1615-9853

e-ISSN

—

Volume of the periodical

19

Issue of the periodical within the volume

21-22

Country of publishing house

US - UNITED STATES

Number of pages

11

Pages from-to

"e1900073"

UT code for WoS article

000492737800001

EID of the result in the Scopus database

2-s2.0-85074554863