IFITM1 as a modulator of surfaceome dynamics and aggressive phenotype in cervical cancer cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F25%3A00080254" target="_blank" >RIV/00209805:_____/25:00080254 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14310/25:00141862

Result on the web

<a href="https://www.spandidos-publications.com/or/53/6/71#" target="_blank" >https://www.spandidos-publications.com/or/53/6/71#</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3892/or.2025.8904" target="_blank" >10.3892/or.2025.8904</a>

Result language

angličtina

Original language name

IFITM1 as a modulator of surfaceome dynamics and aggressive phenotype in cervical cancer cells

Original language description

Interferon-induced transmembrane proteins (IFITMs) are frequently overexpressed in cancer cells, including cervical carcinoma cells, and play a role in the progression of various cancer types. However, their mechanisms of action remain incompletely understood. In the present study, by employing a combination of surface membrane protein isolation and quantitative mass spectrometry, it was comprehensively described how the IFITM1 protein influences the composition of the cervical cancer cell surfaceome. Additionally, the effects of interferon-γ on protein expression and cell surface exposure were evaluated in the presence and absence of IFITM1. The IFITM1-regulated membrane and membrane-associated proteins identified are involved mainly in processes such as endocytosis and lysosomal transport, cell-cell and cell-extracellular matrix adhesion, antigen presentation and the immune response. To complement the proteomic data, gene expression was analyzed using reverse transcription-quantitative PCR to distinguish whether the observed changes in protein levels were attributable to transcriptional regulation or differential protein dynamics. Furthermore, the proteomic and gene expression data are supported by functional studies demonstrating the impact of the IFITM1 and IFITM3 proteins on the adhesive, migratory and invasive capabilities of cervical cancer cells, as well as their interactions with immune cells.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30204 - Oncology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Oncology reports

ISSN

1021-335X

e-ISSN

1791-2431

Volume of the periodical

53

Issue of the periodical within the volume

6

Country of publishing house

GR - GREECE

Number of pages

16

Pages from-to

71

UT code for WoS article

001484857100001

EID of the result in the Scopus database

2-s2.0-105003946572