Systems-level effects of ectopic galectin-7 reconstitution in cervical cancer and its microenvironment

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60162694%3AG44__%2F16%3A43875624" target="_blank" >RIV/60162694:G44__/16:43875624 - isvavai.cz</a>

Result on the web

<a href="https://bmccancer.biomedcentral.com/articles/10.1186/s12885-016-2700-8" target="_blank" >https://bmccancer.biomedcentral.com/articles/10.1186/s12885-016-2700-8</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/s12885-016-2700-8" target="_blank" >10.1186/s12885-016-2700-8</a>

Result language

angličtina

Original language name

Systems-level effects of ectopic galectin-7 reconstitution in cervical cancer and its microenvironment

Original language description

Galectin-7 is negatively regulated in cervical cancer and appears to be a link between apoptotic response triggered by cancer and anti-tumoral activity of the immune system. Our understanding of how cervical cancer cells and their molecular networks adapt in response to the expression of Gal-7 remains limited. Meta-analysis of Gal-7 expression was conducted in three cervical cancer cohort studies and TCGA. In silico prediction and bisulfite sequencing were performed to inquire epigenetic alterations. To study the effect of Gal-7 on cervical cancer we ectopically re-expressed it in HeLa and SiHa cervical cancer cell lines and analyzed their transcriptome and SILAC-based proteome. We also examined the tumor and microenvironment host cell transcriptomes after xenotransplantation into immunocompromised mice. Gal-7 was constantly downregulated in our meta-analysis. Tumors with combined high Gal-7 and low galectin-1 expression presented significantly better prognoses. In silico and bisulfite sequencing assays showed de novo methylation in the Gal-7 promoter and first intron. Cells re-expressing Gal-7 showed a high apoptosis ratio and their xenografts displayed strong growth retardation. Multiple gene modules and transcriptional regulators were modulated in response to Gal-7 reconstitution, both in cervical cancer cells and their microenvironments. Most of these genes and modules were associated with tissue morphogenesis, metabolism, transport, chemokine activity and immune response. These functional modules could exert the same effects in vitro and in vivo, even despite different compositions between HeLa and SiHa samples. Gal-7 re-expression affects the regulation of molecular networks in cervical cancer that are involved in diverse cancer hallmarks such as metabolism, growth control, invasion and evasion of apoptosis. The effect of Gal-7 extends to the microenvironment, where networks involved in its configuration and in immune surveillance are particularly affected.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FD - Oncology and haematology

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

BMC Cancer

ISSN

1471-2407

e-ISSN

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Volume of the periodical

16

Issue of the periodical within the volume

1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

22

Pages from-to

"Art. No. 680"

UT code for WoS article

000384195800001

EID of the result in the Scopus database

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