A protein-specific priority code in presequences determines the efficiency of mitochondrial protein import

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F25%3A00080362" target="_blank" >RIV/00209805:_____/25:00080362 - isvavai.cz</a>

Result on the web

<a href="https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3003298" target="_blank" >https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.3003298</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pbio.3003298" target="_blank" >10.1371/journal.pbio.3003298</a>

Result language

angličtina

Original language name

A protein-specific priority code in presequences determines the efficiency of mitochondrial protein import

Original language description

The biogenesis of mitochondria relies on the import of hundreds of different precursor proteins from the cytosol. Most of these proteins are synthesized with N-terminal presequences which serve as mitochondrial targeting signals. Presequences consistently form amphipathic helices, but they considerably differ with respect to their primary structure and length. Here we show that presequences can be classified into seven different groups based on their specific features. Using a test set of different presequences, we observed that group A presequences endow precursor proteins with improved in vitro import characteristics. We developed IQ-Compete (for Import and de-Quenching Competition assay), a novel assay based on fluorescence de-quenching, to monitor the import efficiencies of mitochondrial precursors in vivo. With this assay, we confirmed the increased import competence of group A presequences. Using mass spectrometry, we found that the presequence of the group A protein Oxa1 specifically recruits the tetratricopeptide repeat (TPR)-containing protein TOMM34 to the cytosolic precursor protein. TOMM34, and the structurally related yeast co-chaperone Cns1, apparently serve as a presequence-specific targeting factor which increases the import efficiency of a specific subset of mitochondrial precursor proteins. Our results suggest that presequences contain a protein-specific priority code that encrypts the targeting mechanism of individual mitochondrial precursor proteins.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10608 - Biochemistry and molecular biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

PLoS biology

ISSN

1544-9173

e-ISSN

1545-7885

Volume of the periodical

23

Issue of the periodical within the volume

7

Country of publishing house

US - UNITED STATES

Number of pages

29

Pages from-to

"e3003298"

UT code for WoS article

001580941900001

EID of the result in the Scopus database

2-s2.0-105011094678