Diversity among wild type and vaccination strains of Trichophyton verrucosum investigated using Random Amplified Polymotphic DNA Analysis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F99%3A00000064" target="_blank" >RIV/00209805:_____/99:00000064 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Diversity among wild type and vaccination strains of Trichophyton verrucosum investigated using Random Amplified Polymotphic DNA Analysis

Original language description

We initially tested 20 primers for their ability to amplify genomic DNA of Trichophyton verrucosum using RAPD. Six of these were selected for further study aimed at discrimination of wild type and vaccination strains of T. verrucosum. The results indicate that RAPD successfully distinguished all strains included in the study. In addition, results of corrected cluster analysis were consistent with the fact that the avirulent vaccination strains (T. verrucosum TV-M9 and T. verr (T. verrucosum TV-M9 and T.verrucosum TV-M-130) were prepared by ultraviolet (UV) light induced mutagenesis of the standard wild type strain T. verrucosum Strážnice. No marker for a/virulence was detected. These outcomes suggest new possibilities for epidemiological analyses, fordiscrimination among different vaccination strains and studies of fungal population in vaccinated/infected hosts.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FD - Oncology and haematology

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

1999

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Folia Biologica

ISSN

0015-5497

e-ISSN

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Volume of the periodical

45

Issue of the periodical within the volume

neuv.

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

6

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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