Novel insights into the assembly and function of human nuclear-encoded cytochrome c oxidase subunits 4, 5a, 6a, 7a and 7b

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F10%3A6854" target="_blank" >RIV/00216208:11110/10:6854 - isvavai.cz</a>

Alternative codes found

RIV/00064165:_____/10:6854

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Novel insights into the assembly and function of human nuclear-encoded cytochrome c oxidase subunits 4, 5a, 6a, 7a and 7b

Original language description

Mammalian CcO (cytochrome c oxidase) is a complex composed of 13 different structural subunits encoded by both the mitochondrial and nuclear genomes. Knockdown of Cox4, Cox5a and Cox6a resulted in reduced CcO activity, diminished affinity of the residualenzyme for oxygen, decreased holoCcO and CcO dimer levels, increased accumulation of CcO subcomplexes and an altered pattern of respiratory supercomplexes. An analysis of the patterns of CcO subcomplexes found in both knockdowns and cells overexpressingepitope-tagged Cox6a, Cox7a and Cox7b identified a novel CcO assembly intermediate, identified the entry points of three late-assembled subunits and demonstrated the interdependence of Cox4 and Cox5a assembly. The ectopic expression of the heart/muscle-specific isoform of the Cox6 subunit (COX6A2) resulted in restoration of both CcO holoenzyme and activity in COX6A1-knockdown cells contrary to the unaltered levels of COX6A2 mRNA in these cells, suggesting the existence of a fixed expres

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biochemical Journal

ISSN

0264-6021

e-ISSN

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Volume of the periodical

428

Issue of the periodical within the volume

part 3

Country of publishing house

GB - UNITED KINGDOM

Number of pages

12

Pages from-to

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UT code for WoS article

000278914400005

EID of the result in the Scopus database

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