Characterization of Dental Pulp Stem Cells from Impacted Third Molars Cultured in Low Serum-Containing Medium

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F11%3A10624" target="_blank" >RIV/00216208:11110/11:10624 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11150/11:10082908 RIV/00179906:_____/11:10082908 RIV/00064165:_____/11:10624

Result on the web

<a href="http://dx.doi.org/10.1159/000321160" target="_blank" >http://dx.doi.org/10.1159/000321160</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Characterization of Dental Pulp Stem Cells from Impacted Third Molars Cultured in Low Serum-Containing Medium

Original language description

We isolated and expanded stem cells from dental pulp from extracted third molars using an innovative culture method consisting of low serum-containing medium supplemented with epidermal growth factor and platelet-derived growth factor BB. We evaluated the differentiation potential of these cells when they were growing either adherently or as micromass/spheroid cultures in various media. Undifferentiated and differentiated cells were analyzed by flow cytometry, immunocytochemistry and immunoblotting. Theflow cytometry results showed that the dental pulp stem cells (DPSCs) were positive for mesenchymal stromal cell markers, but negative for hematopoietic markers. Immunocytochemical and/or immunoblotting analyses revealed the expression of numerous stemcell markers, including nanog, Sox2, nestin, Musashi-1 and nucleostemin, whereas they were negative for markers associated with differentiated neural, vascular and hepatic cells. Surprisingly, the cells were only slightly positive for alp

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FD - Oncology and haematology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cells, Tissues, Organs

ISSN

1422-6405

e-ISSN

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Volume of the periodical

193

Issue of the periodical within the volume

6

Country of publishing house

CH - SWITZERLAND

Number of pages

22

Pages from-to

344-365

UT code for WoS article

000290580200002

EID of the result in the Scopus database

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