Human DPSCs fabricate vascularized woven bone tissue: A new tool in bone tissue engineering

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F17%3A00477268" target="_blank" >RIV/68378041:_____/17:00477268 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11130/17:10373958

Result on the web

<a href="http://dx.doi.org/10.1042/CS20170047" target="_blank" >http://dx.doi.org/10.1042/CS20170047</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1042/CS20170047" target="_blank" >10.1042/CS20170047</a>

Result language

angličtina

Original language name

Human DPSCs fabricate vascularized woven bone tissue: A new tool in bone tissue engineering

Original language description

Human dental pulp stem cells (hDPSCs) are mesenchymal stem cells that have been successfully used in human bone tissue engineering. To establish whether these cells can lead to a bone tissue ready to be grafted, we checked DPSCs for their osteogenic and angiogenic differentiation capabilities with the specific aim of obtaining a new tool for bone transplantation. Therefore, hDPSCs were specifically selected from the stromal-vascular dental pulp fraction, using appropriate markers, and cultured. Growth curves, expression of bone-related markers, calcification and angiogenesis as well as an in vivo transplantation assay were performed. We found that hDPSCs proliferate, differentiate into osteoblasts and express high levels of angiogenic genes, such as vascular endothelial growth factor and platelet-derived growth factor A. Human DPSCs, after 40 days of culture, give rise to a 3D structure resembling a woven fibrous bone. These woven bone (WB) samples were analysed using classic histology and synchrotron-based, X-ray phase-contrast microtomography and holotomography. WB showed histological and attractive physical qualities of bone with few areas of mineralization and neovessels. Such WB, when transplanted into rats, was remodelled into vascularized bone tissue. Taken together, our data lead to the assumption that WB samples, fabricated by DPSCs, constitute a noteworthy tool and do not need the use of scaffolds, and therefore they are ready for customized regeneration.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30211 - Orthopaedics

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Clinical science

ISSN

0143-5221

e-ISSN

—

Volume of the periodical

131

Issue of the periodical within the volume

8

Country of publishing house

GB - UNITED KINGDOM

Number of pages

15

Pages from-to

699-713

UT code for WoS article

000428515000002

EID of the result in the Scopus database

2-s2.0-85017200322