Proliferative potential and phenotypic analysis of long-term cultivated human granulosa cells initiated by addition of follicular fluid

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F11%3A10979" target="_blank" >RIV/00216208:11110/11:10979 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11150/11:10105324 RIV/00179906:_____/11:10105324 RIV/00064165:_____/11:10979
Result on the web
<a href="http://dx.doi.org/10.1007/s10815-011-9617-6" target="_blank" >http://dx.doi.org/10.1007/s10815-011-9617-6</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Proliferative potential and phenotypic analysis of long-term cultivated human granulosa cells initiated by addition of follicular fluid
Original language description
Purpose The aim of this study was to develop and optimize a strategy for long-term cultivation of luteinizing human granulosa cells (GCs). Methods GCs were cultivated in DMEM/F12 medium supplemented with 2% fetal calf serum. In vitro proliferation of GCswas supported by follicular fluid as well as FSH and growth factors. Results The cultured GCs were maintained for 45 days with a doubling time of 159 +/- 24 h. GCs initiated by the addition of follicular fluid and cultivated under low serum conditions reached 10 +/- 0.7 population doublings. GCs maintain the typical phenotypic expression and the telomere length according to specific culture conditions. Conclusion Our present study has demonstrated that GCs can be maintained in vitro for at least 45 days and this cell model can be beneficial when studying hormonal regulation associated with follicular maturation and preparation of oocytes for fertilization.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FK - Gynaecology and obstetrics
OECD FORD branch
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Project
<a href="/en/project/NS9781" target="_blank" >NS9781: Biomarkers of ovarian hyperstimulation syndrome (OHSS) in follicular fluid and plasma/serum of IVF patients - a proteomic study.</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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