Proliferative potential and phenotypic analysis of long-term cultivated human granulosa cells initiated by follicular fluid
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F11%3A39892377" target="_blank" >RIV/00216275:25310/11:39892377 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/s10815-011-9617-6" target="_blank" >http://dx.doi.org/10.1007/s10815-011-9617-6</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10815-011-9617-6" target="_blank" >10.1007/s10815-011-9617-6</a>
Alternative languages
Result language
angličtina
Original language name
Proliferative potential and phenotypic analysis of long-term cultivated human granulosa cells initiated by follicular fluid
Original language description
Purpose: The aim of this study was to develop and optimize a strategy for long-term cultivation of luteinizing human granulosa cells (GCs). Methods: GCs were cultivated in DMEM/F12 medium supplemented with 2% fetal calf serum. In vitro proliferation of GCs was supported by follicular fluid as well as FSH and growth factors. Results: The cultured GCs were maintained for 45 days with a doubling time of 159+-24 hours. GCs initiated by the addition of follicular fluid and cultivated under low serum conditions reached 10+-0.7 population doublings. GCs maintain the typical phenotypic expression and the telomere length according to specific culture conditions. Conclusion: Our present study has demonstrated that GCs can be maintained in vitro for at least 45 days and this cell model can be beneficial when studying hormonal regulation associated with follicular maturation and preparation of oocytes for fertilization.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
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Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Assisted Reproduction and Genetics
ISSN
1058-0468
e-ISSN
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Volume of the periodical
28
Issue of the periodical within the volume
10
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
939-950
UT code for WoS article
000296645200010
EID of the result in the Scopus database
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