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Proliferative potential and phenotypic analysis of long-term cultivated human granulosa cells initiated by follicular fluid

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F11%3A39892377" target="_blank" >RIV/00216275:25310/11:39892377 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.1007/s10815-011-9617-6" target="_blank" >http://dx.doi.org/10.1007/s10815-011-9617-6</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1007/s10815-011-9617-6" target="_blank" >10.1007/s10815-011-9617-6</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Proliferative potential and phenotypic analysis of long-term cultivated human granulosa cells initiated by follicular fluid

  • Original language description

    Purpose: The aim of this study was to develop and optimize a strategy for long-term cultivation of luteinizing human granulosa cells (GCs). Methods: GCs were cultivated in DMEM/F12 medium supplemented with 2% fetal calf serum. In vitro proliferation of GCs was supported by follicular fluid as well as FSH and growth factors. Results: The cultured GCs were maintained for 45 days with a doubling time of 159+-24 hours. GCs initiated by the addition of follicular fluid and cultivated under low serum conditions reached 10+-0.7 population doublings. GCs maintain the typical phenotypic expression and the telomere length according to specific culture conditions. Conclusion: Our present study has demonstrated that GCs can be maintained in vitro for at least 45 days and this cell model can be beneficial when studying hormonal regulation associated with follicular maturation and preparation of oocytes for fertilization.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CB - Analytical chemistry, separation

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2011

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of Assisted Reproduction and Genetics

  • ISSN

    1058-0468

  • e-ISSN

  • Volume of the periodical

    28

  • Issue of the periodical within the volume

    10

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    11

  • Pages from-to

    939-950

  • UT code for WoS article

    000296645200010

  • EID of the result in the Scopus database