DEVELOPMENT OF MONOCLONAL ANTIBODIES SPECIFIC FOR GLYCATED PRION PROTEIN
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F11%3A9526" target="_blank" >RIV/00216208:11110/11:9526 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1080/15287394.2011.618976" target="_blank" >http://dx.doi.org/10.1080/15287394.2011.618976</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
DEVELOPMENT OF MONOCLONAL ANTIBODIES SPECIFIC FOR GLYCATED PRION PROTEIN
Original language description
Transmissive spongiform encephalopathies (TSE) are neurodegenerative diseases characterized by depositions of abnormally folded prion protein (PrP(TSE)) in brain. PrP(TSE) is at present the only specific biochemical marker of human and animal TSE. As deposits of PrP(TSE) remain in the body for long periods, there is substantial chance of them being nonenzymatically modified by glycation. The detection of glycated PrP(TSE) may have potential to serve as a diagnostic marker. Monoclonal antibodies specificfor carboxymethyl lysine/arginine-modified prion protein were prepared. Recombinant human prion protein (rhPrP) was bacterially expressed and purified by affinity chromatography. rhPrP was modified by glyoxylic acid that introduces carboxymethyl groupson lysine and arginine residues present within the molecule of the protein. Modified rhPrP (rhPrP-CML) was used for immunization of 6 mice, and 960 hybridoma cells were prepared. Screening of cell supernatants resulted in the selection of
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Toxicology and Environmental Health. Part A
ISSN
1528-7394
e-ISSN
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Volume of the periodical
74
Issue of the periodical within the volume
22
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
1469-1475
UT code for WoS article
000298322700004
EID of the result in the Scopus database
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