Detection of the GPI-anchorless prion protein fragment PrP226 in human brain

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F13%3A10189589" target="_blank" >RIV/00216208:11110/13:10189589 - isvavai.cz</a>
Alternative codes found
RIV/61389013:_____/13:00398156 RIV/00064190:_____/13:#0000663
Result on the web
<a href="http://dx.doi.org/10.1186/1471-2377-13-126" target="_blank" >http://dx.doi.org/10.1186/1471-2377-13-126</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/1471-2377-13-126" target="_blank" >10.1186/1471-2377-13-126</a>

Result language
angličtina
Original language name
Detection of the GPI-anchorless prion protein fragment PrP226 in human brain
Original language description
Background: The accumulation of the misfolded forms of cellular prion protein, i.e. prions (PrPSc), in the brain is one of the crucial characteristics of fatal neurodegenerative disorders, called transmissible spongiform encephalopathies (TSEs). Cellularprion protein is normally linked to the cell surface by the glycosylphosphatidylinositol (GPI) anchor. There is accumulating evidence that the GPI-anchorless prion protein may act as an accelerator of formation and propagation of prions. In the TSE affected human brain we have previously discovered a novel GPI-anchorless prion protein fragment, named PrP226*, which ends with the tyrosine 226. This fragment can be labeled specifically by the monoclonal antibody V5B2. Methods: We developed a DELFIA basedassay for quick and sensitive detection of the PrP226* fragment in human brain tissue homogenates. By calculating the ratio between the signals of native (N) and denatured (D) samples applied to the assay we were able to observe signific
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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