Induction of the mitochondrial permeability transition (MPT) by micromolar iron: Liberation of calcium is more important than NAD(P)H oxidation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F12%3A12672" target="_blank" >RIV/00216208:11110/12:12672 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11120/12:43907149 RIV/00023752:_____/12:43913774

Result on the web

<a href="http://dx.doi.org/10.1016/j.bbabio.2012.05.008" target="_blank" >http://dx.doi.org/10.1016/j.bbabio.2012.05.008</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Induction of the mitochondrial permeability transition (MPT) by micromolar iron: Liberation of calcium is more important than NAD(P)H oxidation

Original language description

The mitochondrial permeability transition (MET) plays an important role in cell death. The MPT is triggered by calcium and promoted by oxidative stress, which is often catalyzed by iron. We investigated the induction of the MPT by physiological concentrations of iron. Isolated rat liver mitochondria were initially stabilized with EDTA and bovine serum albumin and energized by succinate or malate/pyruvate. The MPT was induced by 20 mu M calcium or ferrous chloride. We measured mitochondrial swelling, theinner membrane potential, NAD(P)H oxidation, iron and calcium in the recording medium. Iron effectively triggered the MPT; this effect differed from non-specific oxidative damage and required some residual EDTA in the recording medium. Evidence in the literature suggested two mechanisms of action for the iron: NAD(P)H oxidation due to loading of the mitochondrial antioxidant defense systems and uptake of iron to the mitochondrial matrix via a calcium uniporter. Both of these events occu

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biochimica et Biophysica Acta - Bioenergetics

ISSN

0005-2728

e-ISSN

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Volume of the periodical

1817

Issue of the periodical within the volume

9

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

13

Pages from-to

1537-1549

UT code for WoS article

000306536400002

EID of the result in the Scopus database

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