Hepcidin Bound to alpha(2)-Macroglobulin Reduces Ferroportin-1 Expression and Enhances Its Activity at Reducing Serum Iron Levels
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F13%3A10189170" target="_blank" >RIV/00216208:11110/13:10189170 - isvavai.cz</a>
Alternative codes found
RIV/00023736:_____/13:00010741
Result on the web
<a href="http://dx.doi.org/10.1074/jbc.M113.471573" target="_blank" >http://dx.doi.org/10.1074/jbc.M113.471573</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1074/jbc.M113.471573" target="_blank" >10.1074/jbc.M113.471573</a>
Alternative languages
Result language
angličtina
Original language name
Hepcidin Bound to alpha(2)-Macroglobulin Reduces Ferroportin-1 Expression and Enhances Its Activity at Reducing Serum Iron Levels
Original language description
Hepcidin regulates iron metabolism by down-regulating ferroportin-1 (Fpn1). We demonstrated that hepcidin is complexedto the blood transport protein, ? 2-macroglobulin (Peslova, G., Petrak, J., Kuzelova, K., Hrdy, I., Halada, P., Kuchel, P. W., Soe-Lin,S., Ponka, P., Sutak, R., Becker, E., Huang, M. L.,Suryo Rahmanto, Y., Richardson, D. R., and Vyoral, D. (2009) Blood 113, 6225-6236). However, nothing is known about the mechanism of hepcidin binding to a 2M or the effects of the ? 2M hepcidin complex in vivo. We show that decreased Fpn1 expression can be mediated by hepcidin bound to native ? 2M and also, for the first time, hepcidin bound to methylamineactivated? 2M (? M-MA). Passage of high molecular weight ? 2M_hepcidin or ? 2M-MA_hepcidin complexes (ALMOST EQUAL TO725 kDa) through a Sephadex G-25 size exclusion column retained their ability to decrease Fpn1 expression. Further studies using ultrafiltration indicated that hepcidin binding to ? 2Mand ? 2M-MA was labile, resulting in
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Biological Chemistry
ISSN
0021-9258
e-ISSN
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Volume of the periodical
288
Issue of the periodical within the volume
35
Country of publishing house
US - UNITED STATES
Number of pages
16
Pages from-to
25450-25465
UT code for WoS article
000330619000041
EID of the result in the Scopus database
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