Hepcidin, the hormone of iron metabolism, is bound specifically to alpha-2-macroglobulin in blood

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F09%3A10000625" target="_blank" >RIV/00216208:11310/09:10000625 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11110/09:3966 RIV/00023736:_____/09:00008180

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Hepcidin, the hormone of iron metabolism, is bound specifically to alpha-2-macroglobulin in blood

Original language description

Hepcidin is a major regulator of iron metabolism. Hepcidin-based therapeutics/diagnostics could play roles in hematology in the future, and thus, hepcidin transport is crucial to understand. In this study, we identify alpha(2)-macroglobulin (alpha(2)-M)as the specific hepcidin-binding molecule in blood. Interaction of I-125-hepcidin with alpha(2)-M was identified using fractionation of plasma proteins followed by native gradient polyacrylamide gel electrophoresis and mass spectrometry. Hepcidin bindingto nonactivated alpha(2)-M displays high affinity (K-d 177 +/- 27 nM), whereas hepcidin binding to albumin was nonspecific and displayed nonsaturable kinetics. Surprisingly, the interaction of hepcidin with activated alpha(2)-M exhibited a classical sigmoidal binding curve demonstrating cooperative binding of 4 high-affinity (K-d 0.3 mu M) hepcidin-binding sites. This property probably enables efficient sequestration of hepcidin and its subsequent release or inactivation that may be imp

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FD - Oncology and haematology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Blood

ISSN

0006-4971

e-ISSN

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Volume of the periodical

113

Issue of the periodical within the volume

24

Country of publishing house

US - UNITED STATES

Number of pages

12

Pages from-to

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UT code for WoS article

000267147100027

EID of the result in the Scopus database

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