Quinacrine reactivity with prion proteins and prion-derived peptides

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F13%3A10189619" target="_blank" >RIV/00216208:11110/13:10189619 - isvavai.cz</a>

Alternative codes found

RIV/61388963:_____/13:00392499

Result on the web

<a href="http://dx.doi.org/10.1007/s00726-013-1460-x" target="_blank" >http://dx.doi.org/10.1007/s00726-013-1460-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00726-013-1460-x" target="_blank" >10.1007/s00726-013-1460-x</a>

Result language

angličtina

Original language name

Quinacrine reactivity with prion proteins and prion-derived peptides

Original language description

Quinacrine is a drug that is known to heal neuronal cell culture infected with prions, which are the causative agents of neurodegenerative diseases called transmissible spongiform encephalopathies. However, the drug fails when it is applied in vivo. In this work, we analyzed the reason for this failure. The drug was suggested to "covalently" modify the prion protein via an acridinyl exchange reaction. To investigate this hypothesis more closely, the acridine moiety of quinacrine was covalently attachedto the thiol groups of cysteines belonging to prion-derived peptides and to the full-length prion protein. The labeled compounds were conveniently monitored by fluorescence and absorption spectroscopy in the ultraviolet and visible spectral regions. Theacridine moiety demonstrated characteristic UV-vis spectrum, depending on the substituent at the C-9 position of the acridine ring. These results confirm that quinacrine almost exclusively reacts with the thiol groups present in proteins

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

<a href="/en/project/GA203%2F07%2F1517" target="_blank" >GA203/07/1517: Chemical syntheses of fluorescence labeled mouse prion proteins using chemical ligations and investigation of their properties</a><br>

Continuities

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Amino Acids

ISSN

0939-4451

e-ISSN

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Volume of the periodical

44

Issue of the periodical within the volume

5

Country of publishing house

AT - AUSTRIA

Number of pages

14

Pages from-to

1279-1292

UT code for WoS article

000317682100004

EID of the result in the Scopus database

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