Comparison of immunohistochemistry, four in situ hybridization methods and quantitative polymerase chain reaction for the molecular diagnosis of HER2 status in gastric cancer: A study of 55 cases

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F14%3A10283497" target="_blank" >RIV/00216208:11110/14:10283497 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11150/14:10283497 RIV/00179906:_____/14:10283497 RIV/00064165:_____/14:10283497

Result on the web

<a href="http://dx.doi.org/10.3892/mmr.2014.2530" target="_blank" >http://dx.doi.org/10.3892/mmr.2014.2530</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3892/mmr.2014.2530" target="_blank" >10.3892/mmr.2014.2530</a>

Result language

angličtina

Original language name

Comparison of immunohistochemistry, four in situ hybridization methods and quantitative polymerase chain reaction for the molecular diagnosis of HER2 status in gastric cancer: A study of 55 cases

Original language description

In the current study, the sensitivity and specificity of methods of HER2 status detection were studied in 55 patients presenting with gastric/gastroesophageal junction carcinoma (30 intestinal and 25 diffuse), in small biopsy (endoscopy; n=33) and resection specimens (n=22). The primary objective of the present study was to compare various methods for the assessment of HER2 status, with regards to the sensitivity and specificity of each method, as well as their concordance. In all cases, the status of HER2 was determined using immunohistochemistry (IHC), fluorescence in situ hybridization (FISH), silver in situ hybridization (SISH), and quantitative polymerase chain reaction (qPCR). The concordance rate between IHC and ISH was 100% for IHC 0 and 3+. The concordance rate for IHC 1+ was 100% between IHC and SISH, and 92.9% between IHC and FISH. The concordance rate among different FISH methods was 100%, between FISH and SISH it was 96.2%, and between qPCR and ISH methods it was 88.5%. Th

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FP - Other medical fields

OECD FORD branch

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Project

<a href="/en/project/LM2010004" target="_blank" >LM2010004: BBMRI_CZ in the building of the Czech part of a large distributed research infrastructure of pan-European importance: the creation and operation of the network of banks of biological material for biomedical research</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Molecular Medicine Reports

ISSN

1791-2997

e-ISSN

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Volume of the periodical

10

Issue of the periodical within the volume

5

Country of publishing house

GR - GREECE

Number of pages

6

Pages from-to

2669-2674

UT code for WoS article

000344424000067

EID of the result in the Scopus database

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