Recruitment of HP1 beta to UVA-induced DNA lesions is independent of radiation-induced changes in A-type lamins

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F14%3A10285499" target="_blank" >RIV/00216208:11110/14:10285499 - isvavai.cz</a>
Alternative codes found
RIV/68081707:_____/14:00428649
Result on the web
<a href="http://dx.doi.org/10.1111/boc.201300076" target="_blank" >http://dx.doi.org/10.1111/boc.201300076</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/boc.201300076" target="_blank" >10.1111/boc.201300076</a>

Result language
angličtina
Original language name
Recruitment of HP1 beta to UVA-induced DNA lesions is independent of radiation-induced changes in A-type lamins
Original language description
Background InformationThe optimal repair of DNA lesions is fundamental for physiological processes. We asked whether the recruitment of HP1, 53BP1 and BMI1 proteins to ultraviolet (UVA)-induced DNA lesions requires functional A-type lamins. ResultsWe found that UVA irradiation of nuclear lamina abolished the fluorescence of mCherry-tagged A-type lamins and destroyed the nuclear lamina as also observed by electron microscopy studies. Similarly, an absence of endogenous A- and B-type lamins was found in irradiated regions by UVA. However, irradiation did not affect the recruitment of HP1, 53BP1 and BMI1 to DNA lesions. The UVA-induced shrinkage of the nuclear lamina, which anchors chromatin, explains why UVA-micro-irradiated chromatin is relaxed. Conversely, additional experiments with -irradiation showed that the nuclear lamina remained intact and the genome-wide level of HP1 was stable. Fluorescence intensity of HP1 and BMI1 in UVA-induced DNA lesions and level of HP1 after -irradiatio
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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