HP1 beta-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F14%3A00441300" target="_blank" >RIV/68081707:_____/14:00441300 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11110/14:10285734
Result on the web
<a href="http://dx.doi.org/10.1186/1756-8935-7-39" target="_blank" >http://dx.doi.org/10.1186/1756-8935-7-39</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/1756-8935-7-39" target="_blank" >10.1186/1756-8935-7-39</a>
Alternative languages
Result language
angličtina
Original language name
HP1 beta-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs
Original language description
Background: The repair of spontaneous and induced DNA lesions is a multistep process. Depending on the type of injury, damaged DNA is recognized by many proteins specifically involved in distinct DNA repair pathways. Results: We analyzed the DNA-damage response after ultraviolet A (UVA) and. irradiation of mouse embryonic fibroblasts and focused on upstream binding factor 1 (UBF1), a key protein in the regulation of ribosomal gene transcription. We found that UBF1, but not nucleolar proteins RPA194, TCOF, or fibrillarin, was recruited to UVA-irradiated chromatin concurrently with an increase in heterochromatin protein 1 beta (HP1 beta) level. Moreover, Forster Resonance Energy Transfer (FRET) confirmed interaction between UBF1 and HP1 beta that was dependent on a functional chromo shadow domain of HP1 beta. Thus, overexpression of HP1 beta with a deleted chromo shadow domain had a dominant-negative effect on UBF1 recruitment to UVA-damaged chromatin.
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
—
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Epigenetics & Chromatin
ISSN
1756-8935
e-ISSN
—
Volume of the periodical
7
Issue of the periodical within the volume
DEC2014
Country of publishing house
GB - UNITED KINGDOM
Number of pages
17
Pages from-to
—
UT code for WoS article
000347931500001
EID of the result in the Scopus database
—