HP1B-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14330%2F14%3A00074061" target="_blank" >RIV/00216224:14330/14:00074061 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1186/1756-8935-7-39" target="_blank" >http://dx.doi.org/10.1186/1756-8935-7-39</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/1756-8935-7-39" target="_blank" >10.1186/1756-8935-7-39</a>
Alternative languages
Result language
angličtina
Original language name
HP1B-dependent recruitment of UBF1 to irradiated chromatin occurs simultaneously with CPDs
Original language description
Background. The repair of spontaneous and induced DNA lesions is a multistep process. Depending on the type of injury, damaged DNA is recognized by many proteins specifically involved in distinct DNA repair pathways. Results. We analyzed the DNA-damage response after ultraviolet A (UVA) and gamma irradiation of mouse embryonic fibroblasts and focused on upstream binding factor 1 (UBF1), a key protein in the regulation of ribosomal gene transcription. We found that UBF1, but not nucleolar proteins RPA194, TCOF, or fibrillarin, was recruited to UVA-irradiated chromatin concurrently with an increase in heterochromatin protein 1-betta (HP1betta) level. Moreover, Förster Resonance Energy Transfer (FRET) confirmed interaction between UBF1 and HP1betta that was dependent on a functional chromo shadow domain of HP1betta. Thus, overexpression of HP1betta with a deleted chromo shadow domain had a dominant-negative effect on UBF1 recruitment to UVA-damaged chromatin.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GBP302%2F12%2FG157" target="_blank" >GBP302/12/G157: Dynamics and Organization of Chromosomes in the Cell Cycle and during Differentiation under Normal and Pathological Conditions</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Epigenetics & Chromatin
ISSN
1756-8935
e-ISSN
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Volume of the periodical
7
Issue of the periodical within the volume
39
Country of publishing house
GB - UNITED KINGDOM
Number of pages
17
Pages from-to
1-17
UT code for WoS article
000347931500001
EID of the result in the Scopus database
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