High density 3D localization microscopy using sparse support recovery

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F14%3A10285732" target="_blank" >RIV/00216208:11110/14:10285732 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1364/OE.22.031263" target="_blank" >http://dx.doi.org/10.1364/OE.22.031263</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1364/OE.22.031263" target="_blank" >10.1364/OE.22.031263</a>

Result language

angličtina

Original language name

High density 3D localization microscopy using sparse support recovery

Original language description

Single-molecule localization microscopy methods offer high spatial resolution, but they are not always suitable for live cell imaging due to limited temporal resolution. One strategy is to increase the density of photoactivated molecules present in eachimage, however suitable analysis algorithms for such data are still lacking. We present 3denseSTORM, a new algorithm for localization microscopy which is able to recover 2D or 3D super-resolution images from a sequence of diffraction limited images withhigh densities of photoactivated molecules. The algorithm is based on sparse support recovery and uses a Poisson noise model, which becomes critical in low-light conditions. For 3D data reconstruction we use the astigmatism and biplane imaging methods. We derive the theoretical resolution limits of the method and show examples of image reconstructions in simulations and in real 2D and 3D biological samples. The method is suitable for fast image acquisition in densely labeled samples and

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

JA - Electronics and optoelectronics

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Optics Express

ISSN

1094-4087

e-ISSN

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Volume of the periodical

22

Issue of the periodical within the volume

25

Country of publishing house

US - UNITED STATES

Number of pages

14

Pages from-to

31263-31276

UT code for WoS article

000346368800118

EID of the result in the Scopus database

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