Separation of replication and transcription domains in nucleoli

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F14%3A10285733" target="_blank" >RIV/00216208:11110/14:10285733 - isvavai.cz</a>
Alternative codes found
RIV/00064165:_____/14:10285733
Result on the web
<a href="http://dx.doi.org/10.1016/j.jsb.2014.10.001" target="_blank" >http://dx.doi.org/10.1016/j.jsb.2014.10.001</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jsb.2014.10.001" target="_blank" >10.1016/j.jsb.2014.10.001</a>

Result language
angličtina
Original language name
Separation of replication and transcription domains in nucleoli
Original language description
In mammalian cells, active ribosomal genes produce the 18S, 5.8S and 28S RNAs of ribosomal particles. Transcription levels of these genes are very high throughout interphase, and the cell needs a special strategy to avoid collision of the DNA polymeraseand RNA polymerase machineries. To investigate this problem, we measured the correlation of various replication and transcription signals in the nucleoli of HeLa, HT-1080 and NIH 3T3 cells using a specially devised software for analysis of confocal images. Additionally, to follow the relationship between nucleolar replication and transcription in living cells, we produced a stable cell line expressing GFP-RPA43 (subunit of RNA polymerase I, pol I) and RFP-PCNA (the sliding clamp protein) based on humanfibrosarcoma HT-1080 cells. We found that replication and transcription signals are more efficiently separated in nucleoli than in the nucleoplasm. In the course of S phase, separation of PCNA and pol I signals gradually increased. During
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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