Gene Expression of Subunits of the IL-12 Family Cytokines in moDCs Derived In Vitro from the Cord Blood of Children of Healthy and Allergic Mothers

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F14%3A10285970" target="_blank" >RIV/00216208:11110/14:10285970 - isvavai.cz</a>

Result on the web

<a href="http://fb.cuni.cz/file/5718/FB2014A0010.pdf" target="_blank" >http://fb.cuni.cz/file/5718/FB2014A0010.pdf</a>

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Gene Expression of Subunits of the IL-12 Family Cytokines in moDCs Derived In Vitro from the Cord Blood of Children of Healthy and Allergic Mothers

Original language description

The incidence of allergic diseases is steadily increasing an urgent need to clarify the immunologic processes which occur early in life and signal an increased risk of possible future allergy development. The ratio and maturation state of DCs together with the cytokine environment are important in directing and modulating immune responses. The maturation state (presence of CD83) of cord blood monocyte-derived dendritic cells (moDCs) 01 52 children of healthy mothers and 58 children of allergic mothers was estimated by flow cytometry. The capacity of moDCs to express genes for subunits of IL-12 family cytokines was monitored using real-time PCR and protein secretion in cell culture supernatants by ELISA. The percentage of CD83(+) moDCs was significantlyhigher in the allergic group after LPS stimulation (43.11 +/- 4.41) in comparison to the healthy group (24.85 +/- 3.37). Significantly higher gene expression of subunits of IL-12 family members was observed in moDCs of children of allerg

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EC - Immunology

OECD FORD branch

—

Project

<a href="/en/project/GD310%2F08%2FH077" target="_blank" >GD310/08/H077: Regulation of immunological mechanisms in health and disease:Development of new diagnostic and therapeutic approaches</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Folia Biologica

ISSN

0015-5500

e-ISSN

—

Volume of the periodical

60

Issue of the periodical within the volume

2

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

9

Pages from-to

74-82

UT code for WoS article

000334986300004

EID of the result in the Scopus database

—