Analysis of 1,2-diol diesters in vernix caseosa by high-performance liquid chromatography - atmospheric pressure chemical ionization mass spectrometry

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F15%3A10292480" target="_blank" >RIV/00216208:11110/15:10292480 - isvavai.cz</a>
Alternative codes found
RIV/61388963:_____/15:00443262 RIV/00216208:11310/15:10292480 RIV/00064165:_____/15:10292480
Result on the web
<a href="http://dx.doi.org/10.1016/j.chroma.2014.11.075" target="_blank" >http://dx.doi.org/10.1016/j.chroma.2014.11.075</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.chroma.2014.11.075" target="_blank" >10.1016/j.chroma.2014.11.075</a>

Result language
angličtina
Original language name
Analysis of 1,2-diol diesters in vernix caseosa by high-performance liquid chromatography - atmospheric pressure chemical ionization mass spectrometry
Original language description
Fatty acid diesters of long-chain 1,2-diols (1,2-DDE), or type II wax diesters, were analyzed in the vernix caseosa of a newborn girl. 1,2-DDE were isolated from the total lipid extract by the semipreparative TLC using plates coated with silica gel. Chromatographic separation of the 1,2-DDE molecular species was achieved on the non-aqueous reversed-phase HPLC with two Nova-Pak C18 columns connected in series (a total length of 45 cm) and using an acetonitrile-ethyl acetate gradient. 1,2-DDE eluted fromthe column in the order of their equivalent chain number. The analytes were detected as ammonium adducts by an ion-trap mass spectrometer equipped with an atmospheric pressure chemical ionization source. Their structures were elucidated using tandem massspectrometry with MS. MS2 and MS3 steps in a data-dependent mode. More than two thousand molecular species of 1,2-DDE were identified in 141 chromatographic peaks. The most abundant 1,2-DDE were monounsaturated lipids consisting of a C22
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Project
<a href="/en/project/GAP206%2F12%2F0750" target="_blank" >GAP206/12/0750: Lipidome of vernix caseosa: comprehensive analysis and biological variability</a><br>
Continuities
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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