Ultrahigh-performance supercritical fluid chromatography for intraclass separation of lipids: Investigation of general principles

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62690094%3A18470%2F22%3A50019225" target="_blank" >RIV/62690094:18470/22:50019225 - isvavai.cz</a>

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S002196732200173X?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S002196732200173X?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chroma.2022.462975" target="_blank" >10.1016/j.chroma.2022.462975</a>

Result language

angličtina

Original language name

Ultrahigh-performance supercritical fluid chromatography for intraclass separation of lipids: Investigation of general principles

Original language description

Powerful chromatographic techniques are required for lipidomic analyses due to the extreme complex-ity of natural lipidomes. In the past few years, ultrahigh-performance supercritical fluid chromatography (UHPSFC) has proven to be a good alternative to conventional LC methods for comprehensive lipidomic analysis. The goal of this work was to study UHPSFC intraclass separation of lipids according to the fatty acyl composition. The effects of column chemistry, mobile phase composition and gradient, flow rate, back pressure, temperature, and column coupling on intraclass separation of lipids were widely inves-tigated and discussed. In general, UHPSFC exhibited interclass selectivity together with intraclass sepa-ration of lipids according to their total number of double bonds and acyl chain lengths. Moreover, sep-arations of diacylglycerol and lysophosphatidylcholines regioisomers were achieved in some cases. The nature of the stationary phase showed the most prominent effect on UHPSFC intraclass selectivity, while other chromatographic conditions were used for partial improvement in resolution of lipid species. An octadecyl-based stationary phase showed excellent separation of nonpolar lipid species, including tria-cylglycerol isobars; however, it provided poor peak shapes and limited retention time reproducibility for polar lipids. Diol-and 1-aminoanthracene-based columns provided the best inter-and intraclass resolu-tion of most lipids. The main benefit for UHPSFC separation of complex lipid samples is the combination of the acyl chain/double bond intraclass separation of lipids with excellent lipid class selectivity, which can facilitate mass spectrometry detection and quantitation of trace species without ion suppression ef-fects.(c) 2022 Elsevier B.V. All rights reserved.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10406 - Analytical chemistry

Project

<a href="/en/project/GA20-12289S" target="_blank" >GA20-12289S: The coupling of supercritical fluid chromatography with mass spectrometry as a new tool for characterization of lipids and polar metabolites</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

1873-3778

Volume of the periodical

1670

Issue of the periodical within the volume

May

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

9

Pages from-to

"Article Number: 462975"

UT code for WoS article

000789608700014

EID of the result in the Scopus database

2-s2.0-85126523436