Column selection for improvement of the UHPLC lipidomic analysis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F23%3A00573205" target="_blank" >RIV/61388963:_____/23:00573205 - isvavai.cz</a>
Result on the web
<a href="https://hdl.handle.net/11104/0343658" target="_blank" >https://hdl.handle.net/11104/0343658</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Column selection for improvement of the UHPLC lipidomic analysis
Original language description
Reversed-phase UHPLC/MS is a widely used lipidomic strategy that allows the separation of the lipid species based on their equivalent carbon number (ECN - the number of carbons minus two times the number of double bonds of all fatty acids). Lipid classes as well as fatty acid compositions are determined using fragments in MSn spectra. Retention time has to correspond with identifying lipid species, so the knowledge of retention dependences significantly enhances the certainty of identification. Increasing chromatographic peak resolution allows identifying more lipid species, and short run times are essential for analyzing large sets of samples in lipidomics. Reducing the slope of the gradient is not a suitable solution for increasing resolution, so selecting a suitable stationary phase is critical. In this study, four C18 columns with sub-2-micrometer particles were compared using a mixture of 71 lipid standards from 16 lipid classes. n
Czech name
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Czech description
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Project
<a href="/en/project/LX22NPO5104" target="_blank" >LX22NPO5104: National Institute for Research of Metabolic and Cardiovascular Diseases</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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