Prolyl Oligopeptidase from the Blood Fluke Schistosoma mansoni: From Functional Analysis to Anti-schistosomal Inhibitors
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F15%3A10295806" target="_blank" >RIV/00216208:11110/15:10295806 - isvavai.cz</a>
Alternative codes found
RIV/60077344:_____/15:00455915 RIV/68378050:_____/15:00446340 RIV/61388963:_____/15:00446340 RIV/00216208:11310/15:10295806
Result on the web
<a href="http://dx.doi.org/10.1371/journal.pntd.0003827" target="_blank" >http://dx.doi.org/10.1371/journal.pntd.0003827</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1371/journal.pntd.0003827" target="_blank" >10.1371/journal.pntd.0003827</a>
Alternative languages
Result language
angličtina
Original language name
Prolyl Oligopeptidase from the Blood Fluke Schistosoma mansoni: From Functional Analysis to Anti-schistosomal Inhibitors
Original language description
Background Blood flukes of the genus Schistosoma cause schistosomiasis, a parasitic disease that infects over 240 million people worldwide, and for which there is a need to identify new targets for chemotherapeutic interventions. Our research is focusedon Schistosoma mansoni prolyl oligopeptidase (SmPOP) from the serine peptidase family S9, which has not been investigated in detail in trematodes. Methodology/Principal Findings We demonstrate that SmPOP is expressed in adult worms and schistosomula in an enzymatically active form. By immunofluorescence microscopy, SmPOP is localized in the tegument and parenchyma of both developmental stages. Recombinant SmPOP was produced in Escherichia coli and its active site specificity investigated using syntheticsubstrate and inhibitor libraries, and by homology modeling. SmPOP is a true oligopeptidase that hydrolyzes peptide (but not protein) substrates with a strict specificity for Pro at P1. The inhibition profile is analogous to those for ma
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FN - Epidemiology, infection diseases and clinical immunology
OECD FORD branch
—
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
PLoS Neglected Tropical Diseases [online]
ISSN
1935-2735
e-ISSN
—
Volume of the periodical
9
Issue of the periodical within the volume
6
Country of publishing house
DE - GERMANY
Number of pages
24
Pages from-to
—
UT code for WoS article
000357398100031
EID of the result in the Scopus database
2-s2.0-84934784341