Expression of the cellular prion protein affects posttransfusion recovery and survival of red blood cells in mice
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F15%3A10313286" target="_blank" >RIV/00216208:11110/15:10313286 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1111/trf.13190" target="_blank" >http://dx.doi.org/10.1111/trf.13190</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/trf.13190" target="_blank" >10.1111/trf.13190</a>
Alternative languages
Result language
angličtina
Original language name
Expression of the cellular prion protein affects posttransfusion recovery and survival of red blood cells in mice
Original language description
BACKGROUNDCellular prion protein (PrPC) is expressed on various cell types including red blood cells (RBCs). The PrPC plays a key role in the pathogenesis of prion diseases, but its physiologic function remains unclear. PrPC is expressed on CD34+ hematopoietic stem cells and its expression is regulated during blood cell differentiation including the erythroid line. STUDY DESIGN AND METHODSWe investigated the role of PrPC in RBC survival in circulation by transfusing a mix of biotin-labeled RBCs from wild-type (WT) and PrP knockout (KO) mice to groups of recipient mice (WT and KO). The proportion of biotinylated RBCs in peripheral blood was estimated by flow cytometry. RESULTSKO RBCs displayed a markedly higher first-day posttransfusion recovery but hada decreased survival in circulation when compared to WT RBCs. Similar results were obtained in all groups of transfused mice, irrespective of RBCs biotinylation level. In addition, we confirmed this finding in an analogous study using Tg
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FD - Oncology and haematology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GAP303%2F12%2F1791" target="_blank" >GAP303/12/1791: The role of proteinase-activated receptors in pathogenesis of prion diseases</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Transfusion
ISSN
0041-1132
e-ISSN
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Volume of the periodical
55
Issue of the periodical within the volume
11
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
2590-2596
UT code for WoS article
000364876100010
EID of the result in the Scopus database
2-s2.0-84947087470