Live imaging reveals spatial separation of parental chromatin until the four-cell stage in Caenorhabditis elegans embryos

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F16%3A10325947" target="_blank" >RIV/00216208:11110/16:10325947 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1387/ijdb.150222cl" target="_blank" >http://dx.doi.org/10.1387/ijdb.150222cl</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1387/ijdb.150222cl" target="_blank" >10.1387/ijdb.150222cl</a>

Result language

angličtina

Original language name

Live imaging reveals spatial separation of parental chromatin until the four-cell stage in Caenorhabditis elegans embryos

Original language description

The parental genomes are initially spatially separated in each pronucleus after fertilization. Here we have used green-to-red photoconversion of Dendra2-H2B-labeled pronuclei to distinguish maternal and paternal chromatin domains and to track their spatial distribution in living Caenorhabditis elegans embryos starting shortly after fertilization. Intermingling of the parental chromatin did not occur until after the division of the AB and P1 blastomeres, at the 4-cell stage. Unexpectedly, we observed that the intermingling of chromatin did not take place during mitosis or during chromatin decondensation, but rather similar to 3-5 minutes into the cell cycle. Furthermore, unlike what has been observed in mammalian cells, the relative spatial positioning of chromatin domains remained largely unchanged during prometaphase in the early C. elegans embryo. Live imaging of photoconverted chromatin also allowed us to detect a reproducible 180 degrees rotation of the nuclei during cytokinesis of the one-cell embryo. Imaging of fluorescently-labeled P granules and polar bodies showed that the entire embryo rotates during the first cell division. To our knowledge, we report here the first live observation of the initial separation and subsequent mixing of parental chromatin domains during embryogenesis.

Czech name

—

Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

—

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Developmental Biology

ISSN

0214-6282

e-ISSN

—

Volume of the periodical

60

Issue of the periodical within the volume

1-3

Country of publishing house

ES - SPAIN

Number of pages

8

Pages from-to

5-12

UT code for WoS article

000375173000002

EID of the result in the Scopus database

2-s2.0-84960463913