The effect of culture medium and carrier on explant culture of human limbal epithelium: A comparison of ultrastructure, keratin profile and gene expression
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F16%3A10330345" target="_blank" >RIV/00216208:11110/16:10330345 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.exer.2016.09.012" target="_blank" >http://dx.doi.org/10.1016/j.exer.2016.09.012</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.exer.2016.09.012" target="_blank" >10.1016/j.exer.2016.09.012</a>
Alternative languages
Result language
angličtina
Original language name
The effect of culture medium and carrier on explant culture of human limbal epithelium: A comparison of ultrastructure, keratin profile and gene expression
Original language description
Patients with limbal stem cell deficiency (LSCD) often experience pain and photophobia due to recurrent epithelial defects and chronic inflammation of the cornea. Successfully restoring a healthy corneal surface in these patients by transplantation of ex vivo expanded human limbal epithelial cells (LECs) may alleviate these symptoms and significantly improve their quality of life. The clinical outcome of transplantation is known to be influenced by the quality of transplanted cells. Presently, several different protocols for cultivation and transplantation of LECs are in use. However, no consensus on an optimal protocol exists. The aim of this study was to examine the effect of culture medium and carrier on the morphology, staining of selected keratins and global gene expression in ex vivo cultured LECs.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FF - ENT (ie. ear, nose, throat), ophthalmology, dentistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/7F14156" target="_blank" >7F14156: Tissue engineering of genetically competent corneal and conjunctival cells for subsequent grafting in human medicine</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Experimental Eye Research
ISSN
0014-4835
e-ISSN
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Volume of the periodical
153
Issue of the periodical within the volume
December
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
122-132
UT code for WoS article
000389287700014
EID of the result in the Scopus database
2-s2.0-84994037274